Three primary advaces in biogas simulation test of "eight five-year plan" key tasks are: (1)The method of suspended inocula was used in the simulation. That is to say

by discarding most of the inactive organic matter of the first time cultured micro-organisms

propagated the active ones and cultured them for the second time so as to improve their density and activity. This method could reduce the biogas produced by inocula themselves and shorten test period: (2) When test temperature increased to 75℃

the bacteria could still be alive and produce methane: (3) The simulation test made on coal and crude oil show that

under anaerobic condition

they can be degraded and can produce methane

and this is also one of the reasons of forming dense oil. What's more

dource produced methane bacteria were obtained from crude oil in this simulation test.