收稿：2019-06-03，
纸质出版：2020-06-28
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油气微生物检测技术因廉价、直接、高效等特点而日益受到油气勘探界的重视。文章对油气勘探潜力较大的顺北地区顺8井北三维区开展了油气微生物异常特征的研究。采取改良平板培养计数法和16S rRNA基因克隆文库法与高通量测序法对土壤样品中的油气微生物进行定量检测，采用改良平板培养计数法了解了顺北地区的微生物数量特征，通过16S rRNA基因克隆文库法与高通量测序法查清了顺北地区油气微生物的种类异常情况。根据检测结果建立了顺北-跃参已知油气藏的微生物数量及种类异常模式，并以此为依据对顺8井北三维区划出了4个微生物数量异常区，推测出未知区存在4个潜在有利勘探目标区（异常区）。
Microbial prospecting of oil and gas (MPOG) is drawing more and more attention of explorationists due to its advantages including low cost
immediacy and high efficiency.Taking the 3D seismic acreage of northern Shun 8 block
Shunbei area
Tarim Basin as the research object
we carried out researches on abnormal characteristics of hydrocarbon-degrading microorganisms
mainly adopting improved plate culture counting method
16S rRNA gene cloning library me-thod and high-throughput sequencing method to quantitatively detect hydrocarbon-degrading microorganisms in soil samples.The former serves to identify the characteristics of microorganisms in respect of quantity in Shunbei area; while the latter two function to ascertain the abnormalities of hydrocarbon-degrading microorganisms in respect of types therein.According to the obtained results
we built models for the abnormal quantity and types of microorganism in the known hydrocarbon reservoirs in Shunbei-Yuecan area
and in turn identified 4 abnormal zones of microbial quantity in 3D seismic acreage of northern Shun 8 block
which are considered to the potential exploration target zones(abnormal zones).
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