Oil & Gas Geology ›› 2020, Vol. 41 ›› Issue (3): 576-585.doi: 10.11743/ogg20200313

• Petroleum Geology • Previous Articles     Next Articles

Microbial characteristics of Shunbei faulted-karst reservoirs and prediction of play fairways,Tarim Basin

Yan Liang1, Ji Miao2, Jia Baoqian2, Chen Xiaotong2, Gao Ping2   

  1. 1. Geophysical Research Institute, SINOPEC, Nanjing, Jiangsu 211100, China;
    2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
  • Received:2019-06-03 Revised:2020-04-23 Published:2020-06-16

Abstract: Microbial prospecting of oil and gas (MPOG) is drawing more and more attention of explorationists due to its advantages including low cost,immediacy and high efficiency.Taking the 3D seismic acreage of northern Shun 8 block,Shunbei area,Tarim Basin as the research object,we carried out researches on abnormal characteristics of hydrocarbon-degrading microorganisms,mainly adopting improved plate culture counting method,16S rRNA gene cloning library me-thod and high-throughput sequencing method to quantitatively detect hydrocarbon-degrading microorganisms in soil samples.The former serves to identify the characteristics of microorganisms in respect of quantity in Shunbei area;while the latter two function to ascertain the abnormalities of hydrocarbon-degrading microorganisms in respect of types therein.According to the obtained results,we built models for the abnormal quantity and types of microorganism in the known hydrocarbon reservoirs in Shunbei-Yuecan area,and in turn identified 4 abnormal zones of microbial quantity in 3D seismic acreage of northern Shun 8 block,which are considered to the potential exploration target zones(abnormal zones).

Key words: plate culture counting method, methane-oxidizing bacteria, butane-oxidizing bacteria, microbial prospecting of oil and gas, Shunbei faulted karst, Tarim Basin

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