塔里木盆地顺北断溶体油气藏微生物特征及有利区预测

doi:10.11743/ogg20200313

摘要/Abstract

摘要： 油气微生物检测技术因廉价、直接、高效等特点而日益受到油气勘探界的重视。文章对油气勘探潜力较大的顺北地区顺8井北三维区开展了油气微生物异常特征的研究。采取改良平板培养计数法和16S rRNA基因克隆文库法与高通量测序法对土壤样品中的油气微生物进行定量检测，采用改良平板培养计数法了解了顺北地区的微生物数量特征，通过16S rRNA基因克隆文库法与高通量测序法查清了顺北地区油气微生物的种类异常情况。根据检测结果建立了顺北-跃参已知油气藏的微生物数量及种类异常模式，并以此为依据对顺8井北三维区划出了4个微生物数量异常区，推测出未知区存在4个潜在有利勘探目标区（异常区）。

关键词: 平板培养法, 甲烷氧化菌, 丁烷氧化菌, 油气微生物勘探, 顺北断溶体, 塔里木盆地

Abstract: Microbial prospecting of oil and gas (MPOG) is drawing more and more attention of explorationists due to its advantages including low cost,immediacy and high efficiency.Taking the 3D seismic acreage of northern Shun 8 block,Shunbei area,Tarim Basin as the research object,we carried out researches on abnormal characteristics of hydrocarbon-degrading microorganisms,mainly adopting improved plate culture counting method,16S rRNA gene cloning library me-thod and high-throughput sequencing method to quantitatively detect hydrocarbon-degrading microorganisms in soil samples.The former serves to identify the characteristics of microorganisms in respect of quantity in Shunbei area;while the latter two function to ascertain the abnormalities of hydrocarbon-degrading microorganisms in respect of types therein.According to the obtained results,we built models for the abnormal quantity and types of microorganism in the known hydrocarbon reservoirs in Shunbei-Yuecan area,and in turn identified 4 abnormal zones of microbial quantity in 3D seismic acreage of northern Shun 8 block,which are considered to the potential exploration target zones(abnormal zones).

Key words: plate culture counting method, methane-oxidizing bacteria, butane-oxidizing bacteria, microbial prospecting of oil and gas, Shunbei faulted karst, Tarim Basin

中图分类号:

TE135

闫亮,季苗,贾宝迁,等. 塔里木盆地顺北断溶体油气藏微生物特征及有利区预测[J]. 石油与天然气地质, 2020, 41(3): 576-585. doi: 10.11743/ogg20200313 Yan Liang,Ji Miao,Jia Baoqian,et al. Microbial characteristics of Shunbei faulted-karst reservoirs and prediction of play fairways,Tarim Basin[J]. Oil & Gas Geology, 2020, 41(3): 576-585. doi: 10.11743/ogg20200313

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